Dataset Information
New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA.
Ontology highlight
ABSTRACT: Introduction
Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species.Methods
We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification.Results
We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species.Conclusions
The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.
SUBMITTER: Julio RGV
PROVIDER: S-EPMC7094048 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA.
Revista da Sociedade Brasileira de Medicina Tropical 20200316
<h4>Introduction</h4>Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species.<h4>Methods</h4>We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification.<h4>Results</h4>We proposed the use of the rpoC gene, encoding the DNA-directed RN ...[more]
