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The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.

ABSTRACT: The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in mammalian cells. Expression of SARS 6 protein in mammalian cells elicits biological activity of stimulating cellular DNA synthesis.

SUBMITTER: Geng H

PROVIDER: S-EPMC7094358 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: expression and functional characterization.

Geng Hua H Liu Yuet-Man YM Chan Wai-Sing WS Lo Anthony Wing-Ip AW Au Deborah Mun-Yee DM Waye Mary Miu-Yee MM Ho Yuan-Yuan YY

FEBS letters 20051121 30

The SARS-CoV open reading frame 6 (ORF6) is transcribed into mRNA6 and encodes a putative 7.5 kDa accessory protein, SARS 6, with unknown function. In this study, we have confirmed the SARS 6 protein expression in lung and intestine tissues of the SARS patients and in SARS-CoV infected Vero E6 cells by immunohistochemistry. Further studies by immunoblot and confocal microscopy analyses revealed the expression and the endoplasmic reticulum (ER) localization of the recombinant SARS 6 protein in ma ...[more]

PMID: 16310783

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