Multiplex molecular detection of respiratory pathogens in children with asthma exacerbation.

ABSTRACT:

Background

Up to 80% of asthma exacerbations in white children are associated with viral upper respiratory infections. The relative importance of different respiratory pathogens and relevant microbiological data in Asian children are unclear. This study elucidated the epidemiology of respiratory infections in Hong Kong children with asthma exacerbation.

Methods

A total of 209 children aged 3-18 years with asthma exacerbations and 77 controls with stable asthma were recruited. The severity of asthma exacerbations was assessed according to Global Initiative for Asthma guideline, and subjects aged 6 years or older performed exhaled nitric oxide and spirometric measurements. Nested multiplex polymerase chain reaction was used to detect 20 different respiratory pathogens.

Resu

SUBMITTER: Leung TF 

PROVIDER: S-EPMC7094527 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature