Unraveling virus identity by detection of depleted probes with capillary electrophoresis.

ABSTRACT: With the emergence of new viral infections and pandemics, there is a need to develop faster methods to unravel the virus identities in a large number of clinical samples. This report describes a virus identification method featuring high throughput, high resolution, and high sensitivity detection of viruses. Identification of virus is based on liquid hybridization of different lengths of virus-specific probes to their corresponding viruses. The probes bound to target sequences are removed by a biotin-streptavidin pull-down mechanism and the supernatant is analyzed by capillary electrophoresis. The probes depleted from the sample appear as diminished peaks in the electropherograms and the remaining probes serve as calibrators to align peaks in different capillaries. The virus identities are

SUBMITTER: Wang CY 

PROVIDER: S-EPMC7094614 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature