An in-silico human cell model reveals the influence of spatial organization on RNA splicing.

ABSTRACT: Spatial organization is a characteristic of all cells, achieved in eukaryotic cells by utilizing both membrane-bound and membrane-less organelles. One of the key processes in eukaryotes is RNA splicing, which readies mRNA for translation. This complex and highly dynamical chemical process involves assembly and disassembly of many molecules in multiple cellular compartments and their transport among compartments. Our goal is to model the effect of spatial organization of membrane-less organelles (specifically nuclear speckles) and of organelle heterogeneity on splicing particle biogenesis in mammalian cells. Based on multiple sources of complementary experimental data, we constructed a spatial model of a HeLa cell to capture intracellular crowding effects. We then developed chemical reactio

SUBMITTER: Ghaemi Z 

PROVIDER: S-EPMC7094823 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature