Identification of <i>slit3</i> as a locus affecting nicotine preference in zebrafish and human smoking behaviour.

ABSTRACT: To facilitate smoking genetics research we determined whether a screen of mutagenized zebrafish for nicotine preference could predict loci affecting smoking behaviour. From 30 screened F₃ sibling groups, where each was derived from an individual ethyl-nitrosurea mutagenized F₀ fish, two showed increased or decreased nicotine preference. Out of 25 inactivating mutations carried by the F₃ fish, one in the slit3 gene segregated with increased nicotine preference in heterozygous individuals. Focussed SNP analysis of the human SLIT3 locus in cohorts from UK (n=863) and Finland (n=1715) identified two variants associated with cigarette consumption and likelihood of cessation. Characterisation of slit3 mutant larvae and adult fish revealed dec

SUBMITTER: Garcia-Gonzalez J 

PROVIDER: S-EPMC7096180 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature