Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues.

ABSTRACT: Understanding the nano-architecture of protein machines in diverse subcellular compartments remains a challenge despite rapid progress in super-resolution microscopy. While single-molecule localization microscopy techniques allow the visualization and identification of cellular structures with near-molecular resolution, multiplex-labeling of tens of target proteins within the same sample has not yet been achieved routinely. However, single sample multiplexing is essential to detect patterns that threaten to get lost in multi-sample averaging. Here, we report maS³TORM (multiplexed automated serial staining stochastic optical reconstruction microscopy), a microscopy approach capable of fully automated 3D direct STORM (dSTORM) imaging and solution exchange employing a re-staining p

SUBMITTER: Klevanski M 

PROVIDER: S-EPMC7096454 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature