Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease.

ABSTRACT:

Background

Liver-type fatty acid-binding protein (L-FABP) is a biomarker for early detection of renal disease in humans. Liver-type fatty acid-binding protein is cytotoxic oxidation products secreted from proximal tubules under ischemia and oxidative stress.

Objective

To examine renal expression and quantify urinary excretion of L-FABP in catswith renal disease.

Animals

One hundred and thirty-four client-owned cats including 34 cats with serum creatinine (sCre) values >1.6 mg/dL and 10 other cats that died in clinics.

Methods

Tissue expressions of L-FABP were examined by reverse transcription polymerase chain reaction and Western blotting. Urinary L-FABP (uL-FABP) and serum L-FABP (sL-FABP) levels were determined by enzyme-linked immunosorbent assay. Anti-liver

SUBMITTER: Katayama M 

PROVIDER: S-EPMC7096645 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature