Tracking the engraftment and regenerative capabilities of transplanted lung stem cells using fluorescent nanodiamonds.

ABSTRACT: Lung stem/progenitor cells are potentially useful for regenerative therapy, for example in repairing damaged or lost lung tissue in patients. Several optical imaging methods and probes have been used to track how stem cells incorporate and regenerate themselves in vivo over time. However, these approaches are limited by photobleaching, toxicity and interference from background tissue autofluorescence. Here we show that fluorescent nanodiamonds, in combination with fluorescence-activated cell sorting, fluorescence lifetime imaging microscopy and immunostaining, can identify transplanted CD45(-)CD54(+)CD157(+) lung stem/progenitor cells in vivo, and track their engraftment and regenerative capabilities with single-cell resolution. Fluorescent nanodiamond labelling did not eliminate the cells

SUBMITTER: Wu TJ 

PROVIDER: S-EPMC7097076 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature