Expression of E93 provides an instructive cue to control dynamic enhancer activity and chromatin accessibility during development.

ABSTRACT: How temporal cues combine with spatial inputs to control gene expression during development is poorly understood. Here, we test the hypothesis that the Drosophila transcription factor E93 controls temporal gene expression by regulating chromatin accessibility. Precocious expression of E93 early in wing development reveals that it can simultaneously activate and deactivate different target enhancers. Notably, the precocious patterns of enhancer activity resemble the wild-type patterns that occur later in development, suggesting that expression of E93 alters the competence of enhancers to respond to spatial cues. Genomic profiling reveals that precocious E93 expression is sufficient to regulate chromatin accessibility at a subset of its targets. These accessibility changes mimic those

SUBMITTER: Nystrom SL 

PROVIDER: S-EPMC7097197 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature