K562 erythroleukemia line as a possible reticulocyte source to culture Plasmodium vivax and its surrogates.

ABSTRACT: Establishing an in vitro "red blood cell matrix" that would allow uninterrupted access to a stable, homogeneous reticulocyte population would facilitate the establishment of continuous, long-term in vitro Plasmodium vivax blood stage cultures. In this study, we have explored the suitability of the erythroleukemia K562 cell line as a continuous source of such reticulocytes and have investigated regulatory factors behind the terminal differentiation (and enucleation, in particular) of this cell line that can be used to drive the reticulocyte production process. The Duffy blood group antigen receptor (Fy), essential for P. vivax invasion, was stably introduced into K562 cells by lentiviral gene transfer. miRNA-26a-5p and miRNA-30a-5p were downregulated to promote erythroid differentiation and

SUBMITTER: Kronstein-Wiedemann R 

PROVIDER: S-EPMC7097847 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature