Electrospun Carbon Nanofibers as an Electrochemical Immunosensing Platform for Vibrio cholerae Toxin: Aging Effect of the Redox Probe.

ABSTRACT: An electrochemical immunosensor for Vibrio cholerae toxin (VCT) has been developed using electrospun carbon nanofibers (CNFs) as the electrode platform. To fabricate the immunosensor, the anti-cholera toxin antibody (Ab) was covalently immobilized on the electrode platforms using the carbodiimide chemistry for the amide bond formation. Every step of the formation of the immunosensor and the subsequent binding of the VCT subunit antigen (Ag) was electrochemically interrogated. The immunosensor gave excellent reproducibility and sensitivities: limits of detection (ca. 1.2 × 10^-13 g mL^-1), limits of quantification (ca. 1.3 × 10^-13 g mL^-1), and a wide linear range for the anti-cholera detection of 8 orders of magnitude (10^-13 to 10<

SUBMITTER: Ozoemena OC 

PROVIDER: S-EPMC7097905 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature