L. pneumophila CMP-5,7-di-N-acetyllegionaminic acid synthetase (LpCLS)-involved chemoenzymatic synthesis of sialosides and analogues.

ABSTRACT: 5,7-Di-N-acetyllegionaminic acid (Leg5,7Ac₂) is a bacterial nonulosonic acid (NulO) analogue of sialic acids, an important class of monosaccharides in mammals and in some bacteria. To develop efficient one-pot multienzyme (OPME) glycosylation systems for synthesizing Leg5,7Ac₂-glycosides, Legionella pneumophila cytidine 5'-monophosphate (CMP)-Leg5,7Ac₂ synthetase (LpCLS) was cloned and characterized. It was successfully used in producing Leg5,7Ac₂-glycosides from chemoenzymatically synthesized Leg5,7Ac₂ using a one-pot two-enzyme system or from its chemically synthesized six-carbon monosaccharide precursor 2,4-diacetamido-2,4,6-trideoxymannose (6deoxyMan2,4diNAc) in a one-pot three-enzyme system. In addition, LpCLS was shown to tolerat

SUBMITTER: McArthur JB 

PROVIDER: S-EPMC7098451 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature