AutoSholl allows for automation of Sholl analysis independent of user tracing.

ABSTRACT:

Background

Sholl analysis has been used to analyze neuronal morphometry and dendritic branching and complexity for many years. While the process has become semi-automated in recent years, existing software packages are still dependent on user tracing and hence are subject to observer bias, variability, and increased user times for analyses. Commercial software packages have the same issues as they also rely on user tracing. In addition, these packages are also expensive and require extensive user training.

New method

To address these issues, we have developed a broadly applicable, no-cost ImageJ plugin, we call AutoSholl, to perform Sholl analysis on pre-processed and 'thresholded' images. This algorithm extends the already existing plugin in Fiji ImageJ for Sholl analysis b

SUBMITTER: Srinivasan A 

PROVIDER: S-EPMC7098465 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature