Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions.

ABSTRACT: We report on the fabrication of bioanodes for H₂ oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H₂ oxidizing currents even under deactivating conditions owing to efficient protection against O₂ combined with a viologen-induced reactivation of the O₂ inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.

SUBMITTER: Ruff A 

PROVIDER: S-EPMC7098691 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature