Anticoagulants Interfere With the Angiogenic and Regenerative Responses Mediated by Platelets.

ABSTRACT:

Background and aims

Platelet rich plasma (PRP) obtained from blood anticoagulated with acid-citrate-dextrose (ACD) or sodium-citrate (SC) is used for regenerative medicine as source of platelet-derived growth factors. Allergic reactions against citrate were reported in patients after local injection of PRP allowing us to hypothesize that anticoagulants exert a harmful and local effect that interferes with the regenerative proprieties of platelets. Herein we test this hypothesis by analyzing the effect of ACD and SC on angiogenic and regenerative responses mediated by platelets.

Methods

PRP was obtained from SC- or ACD-anticoagulated blood; platelets were lysed to release growth factors; and PRP releasates (PRPr) were used to induce in vitro endothelial proliferation a

SUBMITTER: Oneto P 

PROVIDER: S-EPMC7098916 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature