Function-based high-throughput screening for antibody antagonists and agonists against G protein-coupled receptors.

ABSTRACT: Hybridoma and phage display are two powerful technologies for isolating target-specific monoclonal antibodies based on the binding. However, for complex membrane proteins, such as G protein-coupled receptors (GPCRs), binding-based screening rarely results in functional antibodies. Here we describe a function-based high-throughput screening method for quickly identifying antibody antagonists and agonists against GPCRs by combining glycosylphosphatidylinositol-anchored antibody cell display with β-arrestin recruitment-based cell sorting and screening. This method links antibody genotype with phenotype and is applicable to all GPCR targets. We validated this method by identifying a panel of antibody antagonists and an antibody agonist to the human apelin receptor from an immune antibody reper

SUBMITTER: Ren H 

PROVIDER: S-EPMC7099005 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature