Archaeosine Modification of Archaeal tRNA: Role in Structural Stabilization.

ABSTRACT: Archaeosine (G⁺) is a structurally complex modified nucleoside found quasi-universally in the tRNA of Archaea and located at position 15 in the dihydrouridine loop, a site not modified in any tRNA outside the Archaea G⁺ is characterized by an unusual 7-deazaguanosine core structure with a formamidine group at the 7-position. The location of G⁺ at position 15, coupled with its novel molecular structure, led to a hypothesis that G⁺ stabilizes tRNA tertiary structure through several distinct mechanisms. To test whether G⁺ contributes to tRNA stability and define the biological role of G⁺, we investigated the consequences of introducing targeted mutations that disrupt the biosynthesis of G⁺ into the geno

SUBMITTER: Turner B 

PROVIDER: S-EPMC7099136 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature