The vitamin D receptor regulates mitochondrial function in C2C12 myoblasts.

ABSTRACT: Vitamin D deficiency has been linked to a reduction in skeletal muscle function and oxidative capacity; however, the mechanistic bases of these impairments are poorly understood. The biological actions of vitamin D are carried out via the binding of 1α,25-dihydroxyvitamin D₃ (1α,25(OH)₂D₃) to the vitamin D receptor (VDR). Recent evidence has linked 1α,25(OH)₂D₃ to the regulation of skeletal muscle mitochondrial function in vitro; however, little is known with regard to the role of the VDR in this process. To examine the regulatory role of the VDR in skeletal muscle mitochondrial function, we used lentivirus-mediated shRNA silencing of the VDR in C2C12 myoblasts (VDR-KD) and examined mitochondrial respiration and protein content compare

SUBMITTER: Ashcroft SP 

PROVIDER: S-EPMC7099523 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature