Novel approach in whole genome mining and transcriptome analysis reveal conserved RiPPs in Trichoderma spp.

ABSTRACT:

Background

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a highly diverse group of secondary metabolites (SM) of bacterial and fungal origin. While RiPPs have been intensively studied in bacteria, little is known about fungal RiPPs. In Fungi only six classes of RiPPs are described. Current strategies for genome mining are based on these six known classes. However, the genes involved in the biosynthesis of theses RiPPs are normally organized in biosynthetic gene clusters (BGC) in fungi.

Results

Here we describe a comprehensive strategy to mine fungal genomes for RiPPs by combining and adapting existing tools (e.g. antiSMASH and RiPPMiner) followed by extensive manual curation based on conserved domain identification, (comparative) phylogenetic

SUBMITTER: Vignolle GA 

PROVIDER: S-EPMC7099791 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature