Biological potential alterations of migratory chondrogenic progenitor cells during knee osteoarthritic progression.

ABSTRACT:

Background

Although increasing studies have demonstrated that chondrogenic progenitor cells (CPCs) remain present in human osteoarthritic cartilage, the biological alterations of the CPCs from the less diseased lateral tibial condyle and the more diseased medial condyle of same patient remain to be investigated.

Methods

CPCs were isolated from paired grade 1-2 and grade 3-4 osteoarthritic cartilage by virtue of cell migratory capacities. The cell morphology, immunophenotype, self-renewal, multi-differentiation, and cell migration of these CPCs were evaluated. Additionally, the distributions of CD105⁺/CD271⁺ cells in OA osteochondral specimen were determined. Furthermore, a high-throughput mRNA sequencing was performed.

Results

Migratory CPCs (mCP

SUBMITTER: Wang YX 

PROVIDER: S-EPMC7099802 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature