Thyroid hormone receptor beta is critical for intestinal remodeling during Xenopus tropicalis metamorphosis.

ABSTRACT:

Background

Thyroid hormone (T3) is critical for development in all vertebrates. The mechanism underlying T3 effect has been difficult to study due to the uterus-enclosed nature of mammalian embryos. Anuran metamorphosis, which is dependent on T3 but independent of maternal influence, is an excellent model to study the roles of T3 and its receptors (TRs) during vertebrate development. We and others have reported various effects of TR knockout (TRα and TRβ) during Xenopus tropicalis development. However, these studies were largely focused on external morphology.

Results

We have generated TRβ knockout animals containing an out-frame-mutation of 5 base deletion by using the CRISPR/Cas9 system and observed that TRβ knockout does not affec

SUBMITTER: Shibata Y 

PROVIDER: S-EPMC7099810 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature