In vitro cytotoxicity of Auger electron-emitting [⁶⁷Ga]Ga-trastuzumab.

ABSTRACT:

Introduction

Molecular radiotherapy exploiting short-range Auger electron-emitting radionuclides has potential for targeted cancer treatment and, in particular, is an attractive option for managing micrometastatic disease. Here, an approach using chelator-trastuzumab conjugates to target radioactivity to breast cancer cells was evaluated as a proof-of-concept to assess the suitability of ⁶⁷Ga as a therapeutic radionuclide.

Methods

THP-trastuzumab and DOTA-trastuzumab were synthesised and radiolabelled with Auger electron-emitters ⁶⁷Ga and ¹¹¹In, respectively. Radiopharmaceuticals were tested for HER2-specific binding and internalisation, and their effects on viability (dye exclusion) and clonogenicity of HER2-positive HCC1954 and HER2-negativ

SUBMITTER: Othman MFB 

PROVIDER: S-EPMC7099941 | biostudies-literature | 2020 Jan - Feb

REPOSITORIES: biostudies-literature