Meta-SourceTracker: application of Bayesian source tracking to shotgun metagenomics.

ABSTRACT:

Background

Microbial source tracking methods are used to determine the origin of contaminating bacteria and other microorganisms, particularly in contaminated water systems. The Bayesian SourceTracker approach uses deep-sequencing marker gene libraries (16S ribosomal RNA) to determine the proportional contributions of bacteria from many potential source environments to a given sink environment simultaneously. Since its development, SourceTracker has been applied to an extensive diversity of studies, from beach contamination to human behavior.

Methods

Here, we demonstrate a novel application of SourceTracker to work with metagenomic datasets and tested this approach using sink samples from a study of coastal marine environments. Source environment metagenomes were obtained fr

SUBMITTER: McGhee JJ 

PROVIDER: S-EPMC7100590 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature