Genomic analysis of variability in Delta-toxin levels between Staphylococcus aureus strains.

ABSTRACT:

Background

The delta-toxin (δ-toxin) of Staphylococcus aureus is the only hemolysin shown to cause mast cell degranulation and is linked to atopic dermatitis, a chronic inflammatory skin disease. We sought to characterize variation in δ-toxin production across S. aureus strains and identify genetic loci potentially associated with differences between strains.

Methods

A set of 124 S. aureus strains was genome-sequenced and δ-toxin levels in stationary phase supernatants determined by high performance liquid chromatography (HPLC). SNPs and kmers were associated with differences in toxin production using four genome-wide association study (GWAS) methods. Transposon mutations in candidate genes were tested for their δ-toxin levels. We constructed XGBoost mod

SUBMITTER: Su M 

PROVIDER: S-EPMC7100594 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature