METTL3-mediated m⁶A is required for murine oocyte maturation and maternal-to-zygotic transition.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most prevalent epigenetic modification of messenger RNA (mRNA) in higher eukaryotes; this modification is mainly catalyzed by a methyltransferase complex including methyltransferase-like 3 (METTL3) as a key factor. Although m⁶A modification has been proven to play an essential role in diverse biological processes, our knowledge of Mettl3 is still limited because Mettl3 mutations are lethal to embryos in both mammals and plants. In this study, we knocked down Mettl3 by microinjection of its specific short interfering RNAs (siRNAs) or morpholino into fully grown germinal vesicle (GV) oocytes. As a result, we demonstrated that knocking down Mettl3 in female germ cells severely inhibited oocyte maturat

SUBMITTER: Sui X 

PROVIDER: S-EPMC7100890 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature