A virtual reality system to analyze neural activity and behavior in adult zebrafish.

ABSTRACT: Virtual realities are powerful tools to analyze and manipulate interactions between animals and their environment and to enable measurements of neuronal activity during behavior. In many species, however, optical access to the brain and/or the behavioral repertoire are limited. We developed a high-resolution virtual reality for head-restrained adult zebrafish, which exhibit cognitive behaviors not shown by larvae. We noninvasively measured activity throughout the dorsal telencephalon by multiphoton calcium imaging. Fish in the virtual reality showed regular swimming patterns and were attracted to animations of conspecifics. Manipulations of visuo-motor feedback revealed neurons that responded selectively to the mismatch between the expected and the actual visual consequences of motor outpu

SUBMITTER: Huang KH 

PROVIDER: S-EPMC7100911 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature