Fly-QMA: Automated analysis of mosaic imaginal discs in Drosophila.

ABSTRACT: Mosaic analysis provides a means to probe developmental processes in situ by generating loss-of-function mutants within otherwise wildtype tissues. Combining these techniques with quantitative microscopy enables researchers to rigorously compare RNA or protein expression across the resultant clones. However, visual inspection of mosaic tissues remains common in the literature because quantification demands considerable labor and computational expertise. Practitioners must segment cell membranes or cell nuclei from a tissue and annotate the clones before their data are suitable for analysis. Here, we introduce Fly-QMA, a computational framework that automates each of these tasks for confocal microscopy images of Drosophila imaginal discs. The framework includes an unsupervised annotation al

SUBMITTER: Bernasek SM 

PROVIDER: S-EPMC7100978 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature