Development of multiple features of antigen-induced asthma pathology in a new strain of mast cell deficient BALB/c-Kit^W-sh/W-sh mice.

ABSTRACT: Mast cell-deficient mice are widely used to identify and quantify contributions of mast cells to diverse biological responses in vivo, including allergic inflammation. However, despite the fact that scores of genes have been identified as modifiers of allergic inflammation, most mast cell-deficient models have been available only on a single genetic background. We transferred the Kit^W-sh allele onto the BALB/c background to generate BALB/c mast cell-deficient mice (BALB/c-Kit^W-sh/W-sh). BALB/c-Kit^W-sh/W-sh mice have dramatically reduced mast cell numbers (0-2% of wild type) in all tissues examined, as well as subtle hematologic differences from the corresponding wild type mice, including splenomegaly with evidence of increased splenic hematopoiesis. We exam

SUBMITTER: Hernandez JD 

PROVIDER: S-EPMC7102933 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature