2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis.

ABSTRACT: The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2-10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of such distances for a conformational ensemble remains a significant challenge. Using several techniques, we show that electron paramagnetic resonance (EPR) spectroscopy of oligonucleotides spin-labelled with triazole-appended nitroxides at the 2' position offers a robust and minimally perturbing tool for obtaining such measurements. For two nitroxides, we present results from EPR spectroscopy, X-ray crystal structures of B-form spin-labelled DNA duplexes, molecular dynamics si

SUBMITTER: Hardwick JS 

PROVIDER: S-EPMC7102949 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature