Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions.

ABSTRACT: Nucleosomal histones are barriers to the DNA repair process particularly at DNA double-strand breaks (DSBs). However, the molecular mechanism by which these histone barriers are removed from the sites of DNA damage remains elusive. Here, we have generated a single specific inducible DSB in the cells and systematically examined the histone removal process at the DNA lesion. We found that histone removal occurred immediately following DNA damage and could extend up to a range of few kilobases from the lesion. To examine the molecular mechanism underlying DNA damage-induced histone removal, we screened histone modifications and found that histone ADP-ribosylation was associated with histone removal at DNA lesions. PARP inhibitor treatment suppressed the immediate histone eviction at DNA lesio

SUBMITTER: Yang G 

PROVIDER: S-EPMC7102957 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature