A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells.

ABSTRACT: Light-regulated modules offer unprecedented new ways to control cellular behaviour with precise spatial and temporal resolution. Among a variety of bacterial light-switchable gene expression systems, single-component systems consisting of single transcription factors would be more useful due to the advantages of speed, simplicity, and versatility. In the present study, we developed a single-component light-activated bacterial gene expression system (eLightOn) based on a novel LOV domain from Rhodobacter sphaeroides (RsLOV). The eLightOn system showed significant improvements over the existing single-component bacterial light-activated expression systems, with benefits including a high ON/OFF ratio of >500-fold, a high activation level, fast activation kinetics, and/or good adaptability. Ad

SUBMITTER: Li X 

PROVIDER: S-EPMC7102963 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature