CDK-Mediator and FBXL19 prime developmental genes for activation by promoting atypical regulatory interactions.

ABSTRACT: Appropriate developmental gene regulation relies on the capacity of gene promoters to integrate inputs from distal regulatory elements, yet how this is achieved remains poorly understood. In embryonic stem cells (ESCs), a subset of silent developmental gene promoters are primed for activation by FBXL19, a CpG island binding protein, through its capacity to recruit CDK-Mediator. How mechanistically these proteins function together to prime genes for activation during differentiation is unknown. Here we discover that in mouse ESCs FBXL19 and CDK-Mediator support long-range interactions between silent gene promoters that rely on FBXL19 for their induction during differentiation and gene regulatory elements. During gene induction, these distal regulatory elements behave in an atypical manner,

SUBMITTER: Feldmann A 

PROVIDER: S-EPMC7102981 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature