Electrochemical Detection of NT-proBNP Using a Metalloimmunoassay on a Paper Electrode Platform.

ABSTRACT: In this paper, we demonstrate an electrochemical method for detection of the heart failure biomarker, N-terminal prohormone brain natriuretic peptide (NT-proBNP). The approach is based on a paper electrode assembly and a metalloimmunoassay; it is intended for eventual integration into a home-use sensor. Sensing of NT-proBNP relies on the formation of a sandwich immunoassay and electrochemical quantification of silver nanoparticle (AgNP) labels attached to the detection antibodies (Abs). There are four important outcomes reported in this article. First, compared to physisorption of the detection Abs on the AgNP labels, a 27-fold increase in signal is observed when a heterobifunctional cross-linker is used to facilitate this labeling. Second, the assay is selective in that it does not cross-

SUBMITTER: Pollok NE 

PROVIDER: S-EPMC7103555 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature