Regulation of the RNAPII Pool Is Integral to the DNA Damage Response.

ABSTRACT: In response to transcription-blocking DNA damage, cells orchestrate a multi-pronged reaction, involving transcription-coupled DNA repair, degradation of RNA polymerase II (RNAPII), and genome-wide transcription shutdown. Here, we provide insight into how these responses are connected by the finding that ubiquitylation of RNAPII itself, at a single lysine (RPB1 K₁₂₆₈), is the focal point for DNA-damage-response coordination. K₁₂₆₈ ubiquitylation affects DNA repair and signals RNAPII degradation, essential for surviving genotoxic insult. RNAPII degradation results in a shutdown of transcriptional initiation, in the absence of which cells display dramatic transcriptome alterations. Additionally, regulation of RNAPII stability is central to transcription recovery-persiste

SUBMITTER: Tufegdzic Vidakovic A 

PROVIDER: S-EPMC7103762 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature