Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012.

ABSTRACT: One of the major problems of avian infectious bronchitis virus (IBV) is the frequent emergence of new variants. In the present study 205 tracheal swabs and organs were collected from broilers and layers chicken farms during January to August 2012 from 19 governorates all over Egypt. The chickens demonstrated respiratory signs and mortality. Out of the examined samples, 130 of which (about 64%) of suspected farms were positive for IBV with real time RT-PCR. 13 IBV-positive samples were selected for further isolation and characterization. Isolation in specific pathogen free (SPF) embryos was carried out after studies three blind successive passages and the hypervariable region of spike protein1 (SP1) was amplified by RT-PCR and sequenced to study the genetic diversity between the isolated vi

SUBMITTER: Selim K 

PROVIDER: S-EPMC7104145 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature