Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.

ABSTRACT: Matrix vesicles (MVs) are a class of extracellular vesicles that initiate mineralization in cartilage, bone, and other vertebrate tissues by accumulating calcium ions (Ca²⁺) and inorganic phosphate (P_i) within their lumen and forming a nucleation core (NC). After further sequestration of Ca²⁺ and P_i, the NC transforms into crystalline complexes. Direct evidence of the existence of the NC and its maturation have been provided solely by analyses of dried samples. We isolated MVs from chicken embryo cartilage and used atomic force microscopy peak force quantitative nanomechanical property mapping (AFM-PFQNM) to measure the nanomechanical and morphological properties of individual MVs under both mineralizing (+Ca²⁺) and non-mineralizing (-

SUBMITTER: Plaut JS 

PROVIDER: S-EPMC7104627 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature