Copper(II)-binding equilibria in human blood.

ABSTRACT: It has been reported that Cu(II) ions in human blood are bound mainly to serum albumin (HSA), ceruloplasmin (CP), alpha-2-macroglobulin (α2M) and His, however, data for α2M are very limited and the thermodynamics and kinetics of the copper distribution are not known. We have applied a new LC-ICP MS-based approach for direct determination of Cu(II)-binding affinities of HSA, CP and α2M in the presence of competing Cu(II)-binding reference ligands including His. The ligands affected both the rate of metal release from Cu•HSA complex and the value of K_D. Slow release and K_D = 0.90 pM was observed with nitrilotriacetic acid (NTA), whereas His showed fast release and substantially lower K_D = 34.7 fM (50 mM HEPES, 50 mM NaCl, pH 7.4), which was explained with for

SUBMITTER: Kirsipuu T 

PROVIDER: S-EPMC7105473 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature