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Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820.

ABSTRACT: A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (V _max = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity.

SUBMITTER: Kondaveeti S

PROVIDER: S-EPMC7105533 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820.

Kondaveeti Sanath S Patel Sanjay K S SKS Woo Janghun J Wee Ji Hyang JH Kim Sang-Yong SY Al-Raoush Riyadh I RI Kim In-Won IW Kalia Vipin Chandra VC Lee Jung-Kul JK

Indian journal of microbiology 20191126 2

A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The fi ...[more]

PMID: 32255848

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Schizophyllum commune

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