Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell and Testis Development Research.

ABSTRACT:

Background

Human testicular cells are greatly valuable to the research community as tools for studying testicular physiology and the effects of environmental pollutants. Because adult testicular cells have a limited self-organization capacity and life span, we investigated whether human pluripotent stem cells (hPSCs) can be used together with testicular cells to move a step closer toward making an optimal model of the human testis.

Methods

We used in vitro culture of donor testicular cells under serum-containing and chemically defined conditions. CRISPR-Cas9 technology was applied to introduce fluorescent transgenes (mCherry2 and EGFP) into hPSCs and testicular cells. hPSC-derived spheroids were co-cultured with human testicular cells in mini-spin bioreactors.

Results

SUBMITTER: Pryzhkova MV 

PROVIDER: S-EPMC7105586 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature