Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway.

ABSTRACT:

Background

This study aims to investigate the effect of integrin β1 on wound healing induced by adipose-derived stem cells (ADSCs), as well as the corresponding mechanism.

Methods

Integrin β1 was overexpressed in ADSCs. Thereafter, flow cytometry and transwell chambers technology were used to measure the endothelial-like differentiation (CD31 as a biomarker of endothelial cell) and cell migration, respectively. Western blot was used to detect the activation of PI3K/AKT, NF-κB and ERK signaling pathways. The effects of integrin β1 overexpression on healing time, healing rate and fibroblast number were further evaluated in the rat models of chronic refractory wound.

Results

The overexpression of integrin β1 increased CD31⁺ endothelial-like cells (about 3.6-fo

SUBMITTER: Wang Q 

PROVIDER: S-EPMC7105588 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature