Magnetic nanoparticle-based immunoassay for rapid detection of influenza infections by using an integrated microfluidic system.

ABSTRACT: Magnetic manganese ferrite (MnFe2O4) nanoparticles with approximately 100nm in diameter were used to improve the performance of an immunoassay for detecting influenza infections. The synthesized nanoparticles were tested for long-term storage to confirm the stability of their thermal decomposition process. Then, an integrated microfluidic system was developed to perform the diagnosis process automatically, including virus purification and detection. To apply these nanoparticles for influenza diagnosis, a micromixer was optimized to reduce the dead volume within the microfluidic chip. Furthermore, the mixing index of the micromixer could achieve as high as 97% in 2seconds. The optical signals showed that this nanoparticle-based immunoassay with dynamic mixing could successfully achieve a de

SUBMITTER: Hung LY 

PROVIDER: S-EPMC7106285 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature