Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients.

ABSTRACT:

Background

Respiratory tract infections are widespread and may cause significant morbidity and mortality in immunosuppressed populations such as oncological patients.

Objectives

The RealAccurate Respiratory RT PCR Kit covering 14 respiratory viruses was compared to the RespiFinder Smart22, a broad-spectrum multiplex ligation-dependent probe amplification (MLPA) test, targeting 22 viral and bacterial respiratory pathogens.

Study design

After verification of its analytical performance, the clinical performance of the RespiFinder Smart22 was evaluated by re-analysis of 96 respiratory samples from oncological patients. Additionally, the time to result (TTR) of both methods was compared.

Results

The analytical performance of the RespiFinder Smart22 fulfilled all pre

SUBMITTER: Berning L 

PROVIDER: S-EPMC7106480 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature