The stringent response regulator (p) ppGpp mediates virulence gene expression and survival in Erwinia amylovora.

ABSTRACT:

Background

The nucleotide second messengers, i.e., guanosine tetraphosphate and pentaphosphate [collectively referred to as (p) ppGpp], trigger the stringent response under nutrient starvation conditions and play an essential role in virulence in the fire blight pathogen Erwinia amylovora. Here, we present transcriptomic analyses to uncover the overall effect of (p) ppGpp-mediated stringent response in E. amylovora in the hrp-inducing minimal medium (HMM).

Results

In this study, we investigated the transcriptomic changes of the (p) ppGpp⁰ mutant under the type III secretion system (T3SS)-inducing condition using RNA-seq. A total of 1314 differentially expressed genes (DEGs) was uncovered, representing more than one third (36.8%) of all genes in the E. amylovora ge

SUBMITTER: Yang HW 

PROVIDER: S-EPMC7106674 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature