Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades.

ABSTRACT:

Background

Ubiquitination and ubiquitin-like protein post-translational modifications play an enormous number of roles in cellular processes. These modifications are constituted of multistep reaction cascades. Readily implementable and robust methods to evaluate each step of the overall process, while presently limited, are critical to the understanding and modulation of the reaction sequence at any desired level, both in terms of basic research and potential therapeutic drug discovery and development.

Results

We developed multiple robust and reliable high-throughput assays to interrogate each of the sequential discrete steps in the reaction cascade leading to protein ubiquitination. As models for the E1 ubiquitin-activating enzyme, the E2 ubiquitin-conjugating enzyme, the E

SUBMITTER: Fenteany G 

PROVIDER: S-EPMC7106726 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature