Molecular dissection of Secale africanum chromosome 6R^afr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust.

ABSTRACT:

Background

Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6R^afr substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage.

Results

Wheat-S. africanum chromosome 6R^afr deletion and translocation lines were produced and identified by sequential non-denaturing fluorescence in situ hybridization (ND-FISH) using multiple Oligo-based probes. Different ND-FISH patterns were observed between S. cereale 6R and S. africanum 6R^afr. With reference to the physical map of t

SUBMITTER: Li G 

PROVIDER: S-EPMC7106737 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature