Transcriptome Ortholog Alignment Sequence Tools (TOAST) for phylogenomic dataset assembly.

ABSTRACT:

Background

Advances in next-generation sequencing technologies have reduced the cost of whole transcriptome analyses, allowing characterization of non-model species at unprecedented levels. The rapid pace of transcriptomic sequencing has driven the public accumulation of a wealth of data for phylogenomic analyses, however lack of tools aimed towards phylogeneticists to efficiently identify orthologous sequences currently hinders effective harnessing of this resource.

Results

We introduce TOAST, an open source R software package that can utilize the ortholog searches based on the software Benchmarking Universal Single-Copy Orthologs (BUSCO) to assemble multiple sequence alignments of orthologous loci from transcriptomes for any group of organisms. By streamlining search, quer

SUBMITTER: Wcisel DJ 

PROVIDER: S-EPMC7106827 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature