A large-scale comparative study of isoform expressions measured on four platforms.

ABSTRACT:

Background

Most eukaryotic genes produce different transcripts of multiple isoforms by inclusion or exclusion of particular exons. The isoforms of a gene often play diverse functional roles, and thus it is necessary to accurately measure isoform expressions as well as gene expressions. While previous studies have demonstrated the strong agreement between mRNA sequencing (RNA-seq) and array-based gene and/or isoform quantification platforms (Microarray gene expression and Exon-array), the more recently developed NanoString platform has not been systematically evaluated and compared, especially in large-scale studies across different cancer domains.

Results

In this paper, we present a large-scale comparative study among RNA-seq, NanoString, array-based, and RT-qPCR platforms u

SUBMITTER: Zhang W 

PROVIDER: S-EPMC7106849 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature