Antimicrobial peptide and sequence variation along a latitudinal gradient in two anurans.

ABSTRACT:

Background

While there is evidence of both purifying and balancing selection in immune defense genes, large-scale genetic diversity in antimicrobial peptides (AMPs), an important part of the innate immune system released from dermal glands in the skin, has remained uninvestigated. Here we describe genetic diversity at three AMP loci (Temporin, Brevinin and Palustrin) in two ranid frogs (Rana arvalis and R. temporaria) along a 2000 km latitudinal gradient. We amplified and sequenced part of the Acidic Propiece domain and the hypervariable Mature Peptide domain (~ 150-200 bp) in the three genes using Illumina Miseq and expected to find decreased AMP genetic variation towards the northern distribution limit of the species similarly to studies on MHC genetic patterns.

Results

We

SUBMITTER: Cortazar-Chinarro M 

PROVIDER: S-EPMC7106915 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature