Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses.

ABSTRACT:

Background

Transmission of SARS-associated coronavirus (SARS-CoV) is now well controlled, nevertheless, it is important to develop effective methods to identify this virus from other pathogens.

Objectives

The purpose of this study was to identify potential ligands and develop a novel diagnostic test to SARS-CoV using phage display technology.

Study design

The SARS-CoV spike 1 (S1) protein containing the receptor binding region (RBD) was used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After four rounds of biopanning, 10 monoclonal phages with specific binding activity to the S1-RBD protein were obtained and subjected to binding and diagnostic assays.

Results

DNA sequencing showed that two phage displayed p

SUBMITTER: Wang C 

PROVIDER: S-EPMC7108448 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature